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Specifications Polymeric 7.5% cross-linked soft-gel columns for cation, ligand exchange separations of carbohydrates.Compatible with UV and RI detectorsHydrogen form columns for USP L17 applicationsCalcium form columns for USP L19 applicationsLead form columns for USP L34 applicationsOne particle size: 9 μmThree column internal diameters: 4.1, 7.8 and 10.0 mmOne column material: 316 stainless steelThree forms: calcium, lead and hydrogenSeparate compounds through ligand exchange. HC-75 columns separate compounds through ligand exchange. The 8% cross-linked HC-75 uses ligand exchange as the primary mechanism of separation. The different forms of the HC-75 (Hydrogen, Calcium and Lead) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.Pack Your Own Columns - Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.Cation Exchange ColumnsThomas No. 0470U71 HC-75 Calcium 305 X 7.8mmThomas No. 1216M11 HC-75 9µm CAL 250 x 4.1mmThomas No. 1216M12 HC-75 H+F 9µm 305 x 7.8mmStationary PhaseHC-75 (Ca2+)HC-75 (Ca2+)HC-75 (H+)ModeCation ExchangeCation ExchangeCation ExchangeUSP ClassificationUSP L19USP L19USP L17Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size9 µm9 µm9 µmPore SizeGel-TypeGel-TypeGel-TypeFormatColumnColumnColumnHardware Inner Diameter7.8 mm4.1 mm7.8 mmHardware Length305 mm250 mm305 mmHardware MaterialStainless SteelStainless SteelStainless SteelMax. Pressure400 psi400 psi400 psiExchange Capacity5 meq/gm5 meq/gm5 meq/gmMobile Phase Limits100% Water 0-40% Acetonitrile100% Water 0-40% Acetonitrile100% Water 0-40% AcetonitrileBuffer StrengthWaterWater0.0-0.5NTemp Limits25-90°C25-90°C25-90°CRestoration25-90°CFlush with 1% calcium chloride at 0.1 mL/min overnight.Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.Compound ClassificationCarbohydratesCarbohydratesCarbohydratesUOM1 Each1 Each1 EachExchange Capacity/Column32 meq/column12 meq/column32 meq/columnCation Exchange ColumnsThomas No. 1207L06 HC-75 (Pb2+) 9 µm 7.8 x 100 mmThomas No. 1207L07 HC-75 (Pb2+) 9 µm 7.8 x 305 mmThomas No. 1207L08 HC-75 (H+) 9 µm 4.1 x 250 mmStationary PhaseHC-75 (Pb2+)HC-75 (Pb2+)HC-75 (H+)ModeCation ExchangeCation ExchangeCation ExchangeUSP ClassificationUSP L34USP L17USP L17Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size9 µm9 µm9 µmPore SizeGel-TypeGel-TypeGel-TypeFormatColumnColumnColumnHardware Inner Diameter7.8 mm7.8 mm7.8 mmHardware Length100 mm305 mm250 mmHardware MaterialStainless SteelStainless SteelStainless SteelMax. Pressure400 psi400 psi400 psiExchange Capacity5 meq/gm5 meq/gm5 meq/gmMobile Phase Limits100% Water 0-40% Acetonitrile100% Water 0-40% Acetonitrile100% Water 0-40% AcetonitrileBuffer StrengthWaterWater0.0-0.5NTemp Limits25-90°C25-90°C25-90°CRestorationFlush with 1% lead nitrate at 0.1 mL/min overnight.Flush with 1% calcium chloride at 0.1 mL/min overnight.Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.Compound ClassificationCarbohydratesCarbohydratesCarbohydratesUOM1 Each1 Each1 EachExchange Capacity/Column–––Bulk ResinThomas No. 1177G24Thomas No. 1177G25Thomas No. 1177G26Stationary PhaseHC-75 (Ca2+)HC-75 (H+)HC-75 (Pb2+)ModeCation ExchangeCation ExchangeCation ExchangeUSP ClassificationUSP L19USP L17USP L34Particle Size9 µm9 µm9 µmPore SizeGel-TypeGel-TypeGel-TypeFormatBulk ResinBulk ResinBulk ResinExchange Capacity5 meq/gm5 meq/gm5 meq/gmRestorationFlush with 1% calcium chloride at 0.1 mL/min overnight.Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.Flush with 1% lead nitrate at 0.1 mL/min overnight.UOMper gramper gramper gramSemiprep/Preparative Guard ColumnThomas No. 1177G27 (Semiprep/Preparative Guard Column, Hydrogen Form)Thomas No. 1177G28 (Semiprep/Preparative Guard Column, Carbonate Form)Stationary PhasePRP-X400PRP-X500ModeCation ExchangeCation ExchangePacking Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size12-20 µm12-20 µmFormatGuard ColumnGuard ColumnHardware Inner Diameter4.6 mm4.6 mmHardware Length33 mm33 mmGuard Column SizeSemiprep/PreparativeSemiprep/PreparativeHardware MaterialStainless SteelStainless SteelMax. Pressure400 psi400 psiCompound ClassificationCarbohydratesCarbohydratesUOM1 Each1 Each
