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Specifications Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.Ion exclusion for organic acids and alcohols Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes: Hydrogen Bonding —The attraction and retention of sample compounds by the negatively charged sulfonate group. Reversed-Phase —The interaction and retention of the sample compounds by the non-polar polymeric support. Ion Exclusion —The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and nonionic compounds elute later.PXP-300 ColumnsThomas No. 0470V28 Column PRP-X300 7um 250 X 4.1mmThomas No. 1192F07 Column PRP-X300 7um 150 x 4.6mm PEEKThomas No. 0470V27 Column PRP-X300 7um 150 X 4.1mmThomas No. 0470V27 PRP-X300 7 µm 2.1 x 250 mmStationary PhasePRP-X300PRP-X300PRP-X300PRP-X300ModeIon ExclusionIon ExclusionIon ExclusionIon ExclusionUSP ClassificationUSP L22USP L22USP L22USP L22Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size7 µm7 µm7 µm7 µmPore Size100 Å100 Å100 Å100 ÅFormatColumnColumnColumnColumnHardware Inner Diameter4.1 mm4.1 mm4.1 mm2.1 mmHardware Length250 mm150 mm250 mm250 mmHardware MaterialStainless SteelPEEKStainless SteelStainless SteelMax. Pressure5000500050005000Exchange Capacity0.17 meq/gm0.17 meq/gm0.17 meq/gm0.17 meq/gmMobile Phase LimitspH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifierBuffer Strength0.0-0.5N0.0-0.5N0.0-0.5N0.0-0.5NTemp Limits5-60°C5-60°C5-60°C5-60°CRestorationInject several times with 100 µL of 1 N nitric acidInject several times with 100 µL of 1 N nitric acidInject several times with 100 µL of 1 N nitric acidInject several times with 100 µL of 1 N nitric acidCompound ClassificationOrganic Acids/AlcoholsOrganic Acids/AlcoholsOrganic Acids/AlcoholsOrganic Acids/AlcoholsUOM1 Each1 Each1 Each1 EachPXP-X300 Analytical Guard Replacement Cartridges and Starter Kits Thomas No. 1192D97 PRP-X300 Grd Col Rpl Kit (5/PK)Thomas No. 1192D99 PRP-X300 Guard Col Starter KitThomas No. 1192D05 Starter Kit GCII, PRP-X300Stationary PhasePRP-X300PRP-X300PRP-X300ModeIon ExclusionIon ExclusionIon ExclusionPacking Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size12-20 µm12-20 µm12-20 µmFormatReplacement Guard CartridgeGuard Cartridge KitGuard Cartridge KitHardware Inner Diameter2.1 mm2.1 mm4.6 mmHardware Length20 mm20 mm20 mmGuard Column SizeAnalyticalSemiprep/PreparativeSemiprep/PreparativeHardware MaterialStainless SteelStainless SteelStainless SteelMax. Pressure5,000 psi5,000 psi5,000 psiMobile Phase LimitspH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifierTemp Limits––5-60°CCompound ClassificationOrganic Acids/AlcoholsOrganic Acids/AlcoholsOrganic Acids/AlcoholsUOM5/pk1 holder, 2 cartridges1 holder, 1 cartridgePXP-X300 Analytical Guard Replacement Cartridges and Starter KitsThomas No. 1192D79 PEEK Cartridge 5-Pk, PRP-X300Thomas No. 1192D06 Cartridge, GCII, PRP-X300, 2-PkStationary PhasePRP-X300PRP-X300ModeIon ExclusionIon ExclusionPacking Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size12-20 µm12-20 µmFormatReplacement Guard CartridgeReplacement Guard CartridgeHardware Inner Diameter3 mm4.6 mmHardware Length8 mm20 mmGuard Column SizeAnalyticalSemiprep/PreparativeHardware MaterialPEEKStainless SteelMax. Pressure5,000 psi5,000 psiExchange Capacity–0.17 meq/gmMobile Phase LimitspH 1-13. 0-100% aqueous, organic modifierpH 1-13. 0-100% aqueous, organic modifierTemp Limits–5-60°CCompound ClassificationOrganic Acids/AlcoholsOrganic Acids/AlcoholsUOM5/pk1 holder, 2 cartridgesResin PRP-X300Thomas No. 0470U76 Resin PRP-X300 7µmThomas No. 1177G25 Resin PRP-X300 12-20 µmStationary PhasePRP-X300PRP-X300ModeIon ExclusionIon ExclusionParticle Size7 µm12-20 µmPore Size100 Å100 ÅFormatBulk ResinBulk ResinMax. Pressure–5,000 psiExchange Capacity5 meq/gm0.17 meq/gmRestorationFlush with 1% calcium chloride at 0.1 mL/min overnight.Inject several times with 100 µL of 1 N nitric acidUOMper gramper gramSemiprep/Preparative Guard and Analytical Guard Column, PRP-X300Thomas No. 1177F82 Semiprep/Preparative Guard Column, PRP-X300Thomas No. 1177F80 Analytical Guard Column, PRP-X300Stationary PhasePRP-X300PRP-X300ModeIon ExclusionIon ExclusionPacking Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidParticle Size12-20 µm12-20 µmFormatGuard ColumnGuard ColumnHardware Inner Diameter4.6 mm2.1 mmHardware Length33 mm33 mmGuard Column SizeSemiprep/PreparativeAnalyticalHardware MaterialStainless SteelStainless SteelMax. Pressure5,000 psi5,000 psiCompound ClassificationOrganic Acids/AlcoholsOrganic Acids/AlcoholsUOM1 Each1 Each
