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Alexa Fluor™ 546 Carboxylic Acid is a non-reactive form of Alexa Fluor™ 546 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247). Characteristics of Alexa Fluor™ 546 dye include: Laser line: 546 nm Common filter set: TRITC (tetramethylrhodamine) Excitation maxima: 556 nm Emission maxima: 573 nm More About Alexa Fluor™ 546 Dye Alexa Fluor™ 546 dye is a bright, yellow-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. Alexa Fuor™ 546 serves as an alternative for tetramethylrhodamine or Cy™3 dye, with higher initial intensity than Alexa Fluor™ 555 dye, but lower photostability in buffer or antifade. Learn more about this dye and related products. Discover the Benefits of the Alexa Fluor™ Dye Series Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.
