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Alexa Fluor™ 594 Carboxylic Acid is a non-reactive form of Alexa Fluor™ 594 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247). Characteristics of Alexa Fluor™ 594 dye include: Laser line: 561 or 594 nm Common filter set: Texas Red Excitation maxima: 590 nm Emission maxima: 617 nm More About Alexa Fluor™ 594 Dye Alexa Fluor™ 594 dye is a bright, red-fluorescent dye that can be excited using the 561 or 594 nm laser lines. It serves as a direct replacement for Texas Red™ dye with bright signal and excellent photostability, especially in fixed cells. Alexa Fluor™ 594 dye is commonly used with DAPI, and Alexa Fluor™ 488 and 647 dyes for multiplexing. Learn more about this dye and related products. Discover the Benefits of the Alexa Fluor™ Dye Series Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.
