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Alexa Fluor™ 750 Carboxylic Acid is a non-reactive form of Alexa Fluor™ 750 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247). Characteristics of Alexa Fluor™ 750 dye include: Laser line: 750 nm Common filter set: Cy™ 7 Excitation maxima: 749 nm Emission maxima: 775 nm More About Alexa Fluor™ 750 Dye Alexa Fluor™ 750 dye and its conjugates exhibit fluorescence spectra that are very similar to those of CY™ 7 dye. Its fluorescence emission maximum at 775 nm is well separated from commonly used far-red fluorophores such as Alexa Fluor™ 647 or allophycocyanin (APC), facilitating multicolor analysis. Discover the Benefits of the Alexa Fluor™ Dye Series Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.