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Laemmli SDS sample buffer is used for protein sample preparation, which is used in the Laemmli SDS-PAGE system. It is a widely used system for protein electrophoresis. Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins. General • Laemmli SDS sample buffer is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 9% β-mercaptoethanol, 0.03% bromophenol blue• The SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it bindsApplication• Laemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE system• This sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gel• It is also used for polyacrylamide protein gel analysi
