Laemmli SDS sample buffer, reducing (6X), Laemmli SDS sample buffer is used to prepare protein samples in the Laemmli SDS-PAGE system, Each

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For protein sample preparation to be used in the Laemmli SDS-PAGE system. Laemmli SDS sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Further, it is used for polyacrylamide protein gel analysis. General This compound is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 9% β-mercaptoethanol, 0.03% bromophenol blueThe SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it bindsApplicationLaemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE systemThis sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gelIt is also used for polyacrylamide protein gel analysi