RIPA buffer (5X), RIPA buffer, radio immunoprecipitation assay buffer, is used for whole cell isolation of proteins from tissues and cell culture, Each

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RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. It is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. This product is ideal for general cell-tissue lysis and sub cellular organelle or molecular fractionation, including immunoprecipation. General • RIPA can be used for isolation of cytoplasmic, membrane, nuclear, and mitochondrial proteins• Protease inhibitors can be added to RIPA buffer to assist in protein isolationApplication• RIPA buffer is suitable for use in protein isolation from culture cells or homogenized tissues• Downstream applications of RIPA buffer include ELISA, western blot, immunoprecipitation, and SDS-PAGE