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Annexins are a widely expressed family of Ca2 and phospholipid-binding proteins. At least ten have been identified in mammalian tissues. They have also been identified in Drosophila, Hydra, and Dictyostelium. Annexin VI is a 70kDa member of the annexin family. While most of the other annexin family members have a four amino acid sequence repeat, Annexin VI displays an eight residue repeat. It is a widely expressed protein, found in lymphocytes, neurons, and many other cell types. Work on annexins has addressed the possible role of Annexin VI in various stages of the endocytic pathway. Some studies suggest that Annexin VI is required for coated-vesicle budding. However, other research directly contradicts this data. Thus, the role of Annexin VI is still under investigation.Immunofluorescence, Immunoprecipitation, Western Blotting
