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Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cellsTime-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10BacE. coli Competent Cells to form expression bacmidBacmid is then transfected into insect cells for production of recombinant baculovirus particlesEasy colony screeningParent bacmid in DH10BacE. colicontains segment of lacZα genelacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinantsFlexibilityComes with pFastBac1 vector that offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloningTwo other vectors are offered separatelypFastBacHT vector for production of histidine-tagged recombinant protein and pFastBacDual vector for expression of two proteins simultaneouslyRapid purification of recombinant proteinsOptional pFastBacHT vector produces histidine-tagged recombinant protein for rapid protein purification with nickel-chelating resinBaculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysi
