$ 994.08
Details
Utilizes monoclonal antibody specific for human C4a/C4a-desArg coated on 96-well plate. Standards and samples are added to wells, and any C4a-desArg present binds to immobilized antibody. The wells are washed and a mixture of biotinylated polyclonal anti-human C4a antibody and streptavidin-horseradish peroxidase is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and substrate solution is added, which produces a blue color in direct proportion to the amount of C4a-desArg present in initial sample. Stop Solution changes the color from blue to yellow, wells are read at 450nmSolid phase sandwich ELISASignals activation of classical or lectin complement pathways in several autoimmune diseases including rheumatoid arthritis, lupus erythematosus, and acute glomerulonephritis as well as in early stages of organ transplant rejectionsWeak multifunctional proinflammatory mediator by increasing vascular permeability, and to be spasmogenic
