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CAS: 107-43-7, Formula: C₅H₁₁NO₂, Grade/Usage: PCR, MDL: MFCD00012123, Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature., The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures. Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction., 02_BulletPoint: Enhances amplification on GC-rich and difficult DNA targets | Lowers the melting temperature, 13_Caution: For Research Use Only. Not for use in diagnostic procedures., Final SAP Desc Link: 2992793, SAP Description Status: Ready, 001_test: Functional test - 1X Betaine Enchancer Solution is tested for amplification of 613 bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions, 001_result: Passes test, 002_test: Volume check- fill volume >= 0.1 ml per tube, 002_result: Passes test, MW_value: 117.15 g/mol, DESC_CALC_test_BP: Betaine enhancer solution 5M, Storage temperature_value: Freezer, CAS: 107-43-7, Formula: C₅H₁₁NO₂, MDL: MFCD00012123, Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature., The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures. Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction., 02_BulletPoint: Enhances amplification on GC-rich and difficult DNA targets | Lowers the melting temperature, 13_Caution: For Research Use Only. Not for use in diagnostic procedures., Size: 5×1 ml, Final SAP Desc Link: 2992793, SAP Description Status: Ready, 001_test: Functional test - 1X Betaine Enchancer Solution is tested for amplification of 613 bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions, 001_result: Passes test, 002_test: Volume check- fill volume >= 0.1 ml per tube, 002_result: Passes test, MW_value: 117.15 g/mol, DESC_CALC_test_BP: Betaine enhancer solution 5M, Storage temperature_value: Freezer, Cat. No.: 76620-530