CCD 841 CoN cells, Each

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Disease: Normal, Origin: Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking, Species: Homo sapiens, human, Tissue: Colon, Morphology: Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking, Properties: Adherent, Cytogenic data: The line is diploid and no consistent marker chromosomes were observed, Patient: 21 weeks gestation fetus, Medium: AddexBio-formulated EMEM (C0005-01) 10% FBS, Subculture: Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37°C. A subcultivation ratio of 1:2 to 1:3 is recommended. Renew medium once every 3 to 4 days depending on cell density, Freezing Medium: Complete growth medium supplemented with 5% (v/v) DMSO, Biosafety Level: I, Sterility: Bacteria: Negative, Yeast: Negative, Mycoplasma: Negative, Pathogens: HIV: Negative, Hepatitis B: Negative, DNA Profile (STR): Amelogenin: X, CSF1PO: 10,11, D13S317: 11,13, D16S539: 10,11, D5S818:12,13, D7S820: 11, THO1:7,8, TPOX: 9,10, vWA: 14,18, Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: CCD 841 CoN (AddexBio P0009023), References for CCD 841 CoN cells: 1. Thompson, A.A., Dilworth, S. & Hay, R.J. Isolation and culture of colonic epithelial cells in serum-free medium Journal of Tissue Culture Methods (1985) 9: 117.