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Reacts with the human ε-chain, a 20kDa subunit of CD3/T cell antigen receptor complex found on 70%-80% of normal human peripheral blood lymphocytes and 60%-85% of thymocytes. Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjunction with pokeweed mitogen. CD3 plays a role in signal transduction during antigen recognition. UCHT1 antibody stains intracellular CD3 unlike the other CD3 clone, HIT3a, which stains only the extracellular CD3. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.Host Species: MouseClone: UCHT1Isotype: IgG1 κSpecies Reactivity: HumanFlow Cytometry, Functional Assay
