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The RA3-6B2 antibody reacts with an epitope on the extracellular domain of CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B lyphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. Levels of expression of CD45R/B220 on the B-cell lineage appear to be developmentally regulated. It is also found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R/B220 antigen is not on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. CD45R/B220 is commonly used as a pan B-cell marker; however, CD19 expression, detected by mAb 1D3, is reported to be more restricted to the B-cell lineage. mAb RA3-6B2 has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. Cross-reaction of RA3-6B2 mAb with activated human T lymphocytes has been observed. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.Host Species: RatClone: RA3-6B2Isotype: IgG2a κSpecies Reactivity: MouseImmunogen: Mouse Pre-B TumorFlow Cytometry, Immunofluorescence
