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Clone RPA-T8 reacts with the α subunit (32kDa) of the two-chain complex expressed on T-cytoxic/suppressor cell populations. CD8 molecule binds to HLA class I molecules during interaction of CD8 T cells with antigen-presenting cells or with target cells. RPA-T8 reacts with approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. RPA-T8 and HIT8a (Mfr. No. 555337) are not cross-blocking. The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500nm. BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD Horizon V500, visit bdbiosciences.com/colors. When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls. Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.Flow Cytometry
