DpnI (10 U/µL), Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI), Each

Details

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.HighlightsSuperior quality – stringent quality control and industry leading manufacturing processConvenient color-coded Five Buffer SystemIncludes universal Tango buffer for double-digestionsBSA premixed in reaction buffersWide selection of restriction endonuclease specificitiesApplicationsMolecular cloningRestriction site mappingGenotypingSouthern BlotRestriction fragment length polymorphism (RFLP)SNPNoteDpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA.