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In this experiment, students actually purify the restriction enzyme, Eco RI. This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis Fractions that contain Eco RI are identified and pooled Total and specific activities are calculated Recommended for college level courses Experiment times: Packing column, 45 minutes Restriction analysis A, 35 minutes Restriction analysis B, 50 minutes Gel Prep, 30 minutes Electrophoresis, 30 minutes Staining and destaining, two minutes All You Need horizontal gel electrophoresis apparatuspower supplyUV visualization systemwaterbathmicrocentrifugemicrowave or hot plateUV spectrophotometer & cuvettesautomatic micropipet with tipsring stands & clamps10 ml pipetslab glasswareice and ice buckets.Order InfoFor five purificationsRecommended StorageSome components requre freezer storage
