ET-51b( ) DNA Vector, Each

Details

The pET-51b( ) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep·Tag™ II coding sequence that is cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His·Tag™ coding sequence with 10 histidines is compatible with purification, detection, and quantification.