Con A Sepharose 4B Medium, An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids, Each

Details

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activationBinds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groupsGroup-specific adsorbent for molecules containing sugarsConcentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL mediumStable in the pH range 4 to 9, and in the presence of all commonly used buffersAverage particle size: 90μmDelivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol