$ 307.53
Details
The illustra plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.Total preparation time of less than 10 minutes—representing a 50% reduction in protocol timeProduces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencingIncreased ease of use provided minimal changes in pipetting volume from one part of the protocol to the next, less centrifugation steps, color-coded kit components, and lack of organic solventsThe typical plasmid yields from a fresh 1.5 ml culture of E.coli containing a high copy number plasmid is approximately 9 μgCan accommodate high and low copy number plasmid purificatio
