Lentil Lectin Sepharose 4B Medium, Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules, Each

Details

Lentil lectin coupled to Sepharose 4B via cyanogen bromide activationLentil lectin is a metalloprotein, containing Ca2 and Mn2 , which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars Retains binding in 1% deoxycholate; especially suited for purification of detergent-solubilized membrane proteinsLigand concentration 2 mg Lentil lectin/ml; binding capacity for porcine thyroglobulin approx. 16mg/mL mediumAverage particle size 90μmStable in the pH range 3-10, and in the presence of all commonly used buffers (Chelating agents and 8M urea to be avoided)Has more equal affinity for α-D-mannose and α-D-glucose compared to Con AOrder InfoSupplied in 20% ethanol containing 0.9% NaCl, 1mM Ca2 and 1mM Mn2