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Growth-factor Receptor-Bound Protein 2 (GRB2) was isolated by using the EGF receptor C-terminus as a probe in the screening of λgt11 expression libraries. This 24kDa GRB2 protein is ubiquitously expressed and consists of one SH2 domain flanked by two SH3 domains. Its ability to bind proteins through these domains has prompted much investigation of its role as an adaptor protein. Sos, a Ras GDP/GTP exchange protein, is constitutively bound to the SH3 domain of GRB2. Following growth factor stimulation, receptor tyrosine kinases autophosphorylate, creating a binding site for the GRB2 SH2 domain. Alternatively, GRB2 interacts with the receptor-bound active Shc protein.Through these interactions, the GRB2/Sos complex is translocated to the membrane where Sos activates membrane-bound Ras to initiate the Ras signaling pathway. Other proteins that similarly interact with GRB2 include Cbl, PTP1D, and Dynamin. However,the mechanisms through which these associations impact cellular responses remain to be discovered.Host Species: MouseClone: 81Isotype: IgG1Species Reactivity: RatImmunogen: Rat GRB2 aa. 1-217Formula Weight [Chemical]: 24kDaBioimaging, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
