Ham's F-12 Nutrient Mix, GlutaMAX Supplement, Designed for serum-free single-cell plating of Chinese hamster ovary cells, Case of 10

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Designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cellsF-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cellsThis Ham's F-12 supplement is modified with GlutaMAX™ and phenol red indicatorCompared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidineSerum-free growth of CHO cells in F-12 has led to a variety of improved formulationsIt minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use formatGibco™ F-12 contains no proteins or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pHStore at 2°C to 8°C, protected from light