Ham's F-12K (Kaighn's) Medium, A modification of Ham's F-12 Nutrient Mixture, Case of 10

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Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) Medium is modified as follows: with L-glutamine, phenol red; without HEPES.Contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvateAdditions allow medium to be supplemented with very low levels of serum or defined components, for some cell typesContains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS)FBS concentration must be optimized for each cell lineUses sodium bicarbonate buffer system (2.5g/L), and therefore requires 5-10% CO2 environment to maintain physiological pHSustainable packagingApplications:Cell Culture, Mammalian Cell Culture