HEPES (1M), A zwitterionic organic chemical buffering agent commonly used in cell culture media, Case of 20

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HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture mediaThe addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubatorA good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stableIt also has very low visible and UV light absorbanceStore at 2°C to 8°C