High-Capacity cDNA Reverse Transcription Kit, Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA, Each

Details

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.Linear target amplification for real-time PCRHigher yields and precision than other cDNA synthesis kits at a fraction of the costTen-fold greater dynamic range than other kitsOptimal reaction temperature: 37°CFinal product size: 7kb or lessExtensively tested with a variety of templatesQuantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNAPCR & Real-Time PCR, Reverse Transcriptio