High-Capacity cDNA Reverse Transcription Kit, Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA, Each

Details:

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.Linear target amplification for real-time PCRHigher yields and precision than other cDNA synthesis kits at a fraction of the costTen-fold greater dynamic range than other kitsOptimal reaction temperature: 37°CFinal product size: 7kb or lessExtensively tested with a variety of templatesQuantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNAPCR & Real-Time PCR, Reverse Transcriptio