HinfI, HC (50 U/µL), The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer, Each

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Lambda DNA digested with HinfI, 1.4% agarose, 148 cleavage sitesconventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'...G▵A N T C...3'3'...C T N A▵G...5' Conditions for 100% Activity: 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA Incubate at 37°CStorage Buffer: HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolMethylation Effects: Dam: never overlaps —no effect Dcm: never overlaps —no effect CpG: may overlap —cleavage impaired EcoKI: never overlaps —no effect EcoBI: may overlap —blockedCompatible Ends: G▴AWTC — PfeI