$ 659.68
Details
Thermo Scientific™ KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5′-termini concentration of 0.02μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with KpnI.5'..G G T A C▵C...3'3'..C▵C A T G G...5'Conditions for 100% Activity:1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/mL BSAIncubate at 37°CStorage Buffer:KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolMethylation Effects:Dam: never overlaps —no effectDcm: may overlap —no effectCpG: may overlap —no effectEcoKI: never overlaps —no effectEcoBI: never overlaps —no effectDigestion of Agarose-embedded DNA:Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hoursNote:High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
