Lambda DNA/EcoRI Marker, Perform sizing and approximate quantification of large double-stranded DNA fragments in agarose gels, Each

Details

Lambda DNA is digested to completion with the appropriate Thermo Scientitific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Recources for Protocols).HighlightsSizing and approximate quantification of large DNA fragmentsSharp bandsSupplied with loading dye for sample DNA