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Specifications Synonym: Phaseolus vulgaris agglutinin, PHAEC Number: 232-718-7Analysis Note Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.Biochem/physiol Actions PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.Product LineBioReagentFormlyophilized powderPotency<40 μg per mL agglutination activityImpuritiessalt, freeSolubilityPBS: soluble2 mg/mLSuitabilitysuitable for cell cultureStorage Temp.2-8°C
