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The Levonorgestrel (LNG) ELISA quantitates LNG in serum,plasma,urine,dried fecal extracts,saliva,milk,or cell culture medium.Principle of the methodThe Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum,plasma,saliva,urine,dried fecal samples,milk,and tissue culture media samples. This kit measures total LNG in extracted serum,plasma,milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm. Rigorous validationEach manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.
