tRNA for LightShift Chemiluminescent RNA EMSA Kit, Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays, Each

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Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction. Highlights:Sensitive – chemiluminescent detection comparable to radioactive detectionTime-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systemsFlexible – compatible with RNA probes biotinylated by different methodsEasy to use – complete kit includes optimized reagents for binding reactions and RNA probe detectionNon-radioactive – eliminate waste concerns from radioactive RNA probe