Live and Dead Cell Assay

Details

ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy. The provided Live and Dead Assay stain is sufficient for ~1000 assays.The Live and Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.The Live and Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.Store the Live and Dead staining solution at -20°C. Allow the product to warm to room temperature before opening. Use diluted solutions of Live/Dead stain promptly.