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The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the “lineage cocktail” used in studies of hematopoietic lineages. The mAb 1A8 (Mfr. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.Host Species: RatClone: RB6-8C5Isotype: IgG2b, κSpecies Reactivity: MouseImmunogen: Not ReportedFlow Cytometry
