Medium 199, Earle's Salts, Originally developed for nutritional studies of chick embryo fibroblasts, Case of 10

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Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. This M199 is modified as follows: with phenol red, L-glutamine; without HEPES.Contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitaminsAvailable with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2Contains no proteins, lipids, or growth factorsUses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pHSustainable packagingCell Culture, Mammalian Cell Culture