Hyaluronidase, Streptomyces hyalurolyticus, Source: Streptomyces hyalurolyticus, Each

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Hyaluronidase is the enzyme that degrades hyaluronic acid in mucopolysaccharides to low molecular saccharides. Hyaluronidase purified from Streptomyeshyalurolyticus is distinct from the known hyaluronidase in its substrate specificity and mechanism of action. This enzyme is quite specific for hyaluronic acid. Various types of chondroitin sulfate (A,B,C,D,,E), chondroitin, heparin, keratan sulfate and chitin are not attacked by this enzyme. This enzyme splits the N-acetylglucosaminic linkages in hyaluronic acid eliminatively , yielding ▿-4,5-unsaturated tetrasaccharides and hexasaccharides as the end-product. Formula Weight: 55kDaCAS #: 37326-33-3Synonyms: EC 4.2.2.1; Hyaluronate lyase; MucinasePhysical Appearance : White lyophilized powderTypical : ã 2,000 TRU/mg proteinContaminants : Protease activity: less than 0.05 u/ampule. One unit is the amount of protease activity which produces 100 g of tyrosine from milk casein at 37°C for 60 min. (pH 6.0)Optimum pH: 6.0Recommended reaction temperature : 60°CMolecular Weight : 91,000 (gel filtration)Inhibitors : Mn2 , Hg2 , Potassium ferricyanideUnit Definition : One TRU (Turbidity Reducing Unit) is that amount of enzyme which causes 50% of decrease in absorbance at 660 nm in 30 minutes at 60°C.