$ 174.80
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SUPER-COMP is an extremely rich medium that significantly increases the competency of the cells when using a simple RbCl/CaCl means of preparing competent host cells.Dilute 1mL of a fresh overnight culture of host cells grown in LB medium into 25mL of 37°C SUPER-COMP media in a 500mL flaskIncubate cells with vigorous aeration for 1-2 hours until cells reach mid-log phase (approximately 8 x 108 cells/mL, OD600 reading 0.8 for most E. coli host cells)Centrifuge culture in a sterile tube at 4°C to pellet cells. Resuspend pellet in 15mL of ice cold 1x RbCl/CaCl Transformation SaltsAfter 15 min. on ice, centrifuge to pellet cells and gently resuspend in 2mL of ice cold 1x RbCl/CaCl Transformation Salts; Keep cell suspension on iceCells are now competent and are immediately ready for DNA uptake. For maximum transformation efficiency, store for 12-24 hours at 4°C to increase competency 4-6 fold (at 48 hours they will be approximately as efficient as when first made). Competent cells can also be frozen in aliquots at -80°C after adding glycerol to 20%.
