SD MEDIUM-LEU-TRP, Powder, Synthetic defined yeast media with all necessary components for selective growth of Saccharomyces cerevisiae, Each

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Each SD or SDA formulation contains the appropriate CSM-based amino acid mixture and 1.7g/liter YNB, 5g/liter Ammonium Sulfate, and 20g/liter Dextrose. SDA formulations also contain 17g/L Agar YDouble dropout formulationPouchesFormulationAdd contents of the pouch (13.7g) to 500mL of distilled, deionized waterAdd agar 17g/L if pouring platesAutoclave at 121°C for 15 minutesYeast cell culture, Gene expression, Transcription regulatio