Gel Loading Dye, Purple (6X), no SDS – 4 ml, Each

Details

Gel Loading Dye, Purple (6X), no SDS - 4.0 ml. Gel Loading Dye, Purple (6X), no SDS is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band is the major indicator and migrates similarly to Bromophenol Blue on agarose gels. Specifically chosen, this dye does not leave a shadow under UV light. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes.