NheI (10 U/µL), The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI), Each

Details

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.HighlightsSuperior quality – stringent quality control and industry leading manufacturing processConvenient color-coded Five Buffer SystemIncludes universal Tango buffer for double-digestionsBSA premixed in reaction buffersWide selection of restriction endonuclease specificitiesApplicationsMolecular cloningRestriction site mappingGenotypingSouthern BlotRestriction fragment length polymorphism (RFLP)SNPNoteLow salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity. NheI is inhibited by salt concentrations above 100 mM. Supercoiled plasmids may require up to 10-fold more NheI for complete digestion than linear DNAs (e.g. 10 units are required to cleave 1 µg of.)