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Ninjurin is a protein whose expression is dramatically increased after sciatic nerve transection and crush injuries. The Ninjurin gene encodes for a protein of 152 amino acids with two putative transmembrane domains. Ninjurin was iodinated in vivo and promoted the aggregation of Jurkat cells expressing Ninjurin, indicating a portion of Ninjurin is exposed to the cell surface. The adhesive properties of Ninjurin were energy and temperature dependent, required Ca2 ; and Mg2 , and the integrity of the cytoskeleton. Also, the adhesion domain of Ninjurin is located at the extracellular NH2-terminal domain. Although its predicted mass is 16kDa, Ninjurin migrates as a 18-22kDa protein in SDS-PAGE, depending on the cell line or tissue. mRNA analysis revealed that Ninjurin is widely expressed with the highest levels in liver, thymus, heart, and the lowest level in brain.Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
