$ 472.72
Details
Thermo Scientific™ NotI is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 95% of the digested DNA fragments can be ligated and recut at a 5′-termini concentration of 0.5μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with NotI.5'..G C▵G G C C G C...3'3'..C G C C G G▵C G...5'Conditions for 100% Activity:1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSAIncubate at 37°CStorage Buffer:NotI is supplied in: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and Recleavage:After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recutMethylation Effects:Dam: never overlaps —no effectDcm: never overlaps —no effectCpG: completely overlaps —blockedEcoKI: never overlaps —no effectEcoBI: never overlaps —no effectDigestion of Agarose-embedded DNA:Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hoursCompatible Ends:Bsp120I, CfrI, Eco52I Note:Assayed using pTZ19RJL2 plasmid DNA digested with BseLI. Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.
