OPTIZYME RsaI, Fisher BioReagents, Each

Details

5'...G T^A C...3'3'...C A^T G...5'Supplied with: 10X OPTIZYME Buffer 4Conditions for 100% Activity:1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSAIncubate at 37°CEnzyme Activity in OPTIZYME buffers:Buffer 1: 50 - 100%Buffer 2: 20 - 50%Buffer 3: 0 - 20%Buffer 4: 100%Buffer 5: 0 - 20%Storage Buffer:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerolLigation and Re-cleavage:More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with RsaIMethylation Effects:Dam: Never overlaps - no effectDcm: Never overlaps - no effectCpG: May overlap - cleavage impaired5'...GTAm5C G...3'3'...CAT Gm5C...5' (Cleavage is impaired)5'...m5C GTAm5C G...3'3'... Gm5CAT Gm5C...5' (Cleavage is blocked)EcoKI: Never overlaps - no effectEcoBI: Never overlaps - no effect