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Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple α-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3MDa-complex that includes γ-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and γ-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and γ-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity.Host Species: MouseClone: 30Isotype: IgG1Species Reactivity [for Features Main]: HumanImmunogen: Mouse pericentrin aa. 1692-1814Immunofluorescence, Western Blotting
