Pierce BCA Protein Assay Reagent A, Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm, Each

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Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide. Additional Information and Online Ordering »A component of the Pierce BCA Protein Assay KitKit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standardPierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein researchThe Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applicationsCompared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracyEstimate visually or measure with a standard spectrophotometer or plate reader at 562 nmExhibits less protein-to-protein variation than dye-binding methods (Bradford)Unaffected by typical concentrations of most ionic and non-ionic detergentsAssay has a 30 min. incubation; much easier and four times faster than classical Lowry methodsLinear working range for BSA of 20 to 2000 µg/mLDetect down to 5 µg/mL with the enhanced protocolStore at room temperature