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Prostasin is a serine protease expressed as both GPI-anchored and secreted forms. Prostasin displays trypsin-like enzymatic activity by hydrolyzing substrates, such as D-Pro-Phe-Arg-AMC. This activity is inhibited by aprotinin, antipain, leupeptin, and benzamidine. The highest levels of prostasin protein are found in semen and the prostate, but many other tissues express prostasin at low levels. The sequence of prostasin is similar to other proteases, such as prostase, testisin, plasma kallikrein, and acrosin. In seminal fluid, prostasin can be found in complex with prostasin-binding protein, which can inhibit prostasin enzymatic activity. Prostasin mRNA is found in normal prostate epithelial cells and non-invasive prostate cancer cells, but is not expressed in invasive prostate cancer cell lines. Expression of prostasin in the invasive prostate cancer cell lines, DU-145 and PC-3, reduces the invasiveness of the cells in vitro. Thus, prostasin is a serine protease that may have roles in normal prostate function and in suppression of tumor cell invasion.Western Blotting
