Purification Of Green Fluorescent Protein S,8 Station Kit, Kit

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Intermediate–Easy To Perform; Requires Some Background Knowledge.Bring Genetic Engineering Into Your Classroom With The Green Gene Colony Transformation . Developed With The Dna Learning Center, This Allows Students To Induce Bacteria To Accept And Express The Gene For Green Fluorescent Protein (Gfp) From Sea Jellies Causing The Genetically Modified Bacteria They Produce To Visibly Glow Green. Includes A Voucher To Request Perishable Materials Later At Your Convenience. Contact Us Or Return The Voucher To Request Delivery Of Perishable Materials.This Is Module 1 Of A 3-Module Sequence And Provides The Materials Necessary For Performing Transformation. To Perform Protein Purification After Completing This Experiment, Obtain Module 2 (Item #211072P Or #211072), And To Run Electrophoresis On The Purified Protein, Obtain Module 3 (Item #211073P Or #211073).Ap Is A Trademark Registered And/Or Owned By The College Board, Which Was Not Involved In The Production Of, And Does Not Endorse, This Product.Product Featuresgreen Color Is Visible In Normal Light–No Uv Lighting Neededpre-Aliquoted And Prepared Reagents Minimize Teacher Prep Timedesigned To Match The Traditional Ap Biology Lab 6Colonies Will Fluoresce Under Uv Lightdesigned For 8 Student Groups/Lab Stationsplasmid Contains Gene For Ampicillin Resistancetime Requirementteacher Prep, Prepare Lb Agar Plates And Streak Start Plates. Performing Lab, Approximately One To Two 60-Minute Class Periods. Post Lab Results, Approximately One 60-Minute Class Period.Digital Resourcesincludes 1-Year Access To Digital Resources That Include A Teacher–S Manual, Student Guide, Fill-In Answer Sheets, Whiteboard Resources, Videos, Interactive Digital Prelab Activity, And More.Performance Expectation(S)Hs-Ls1-1Hs-Ls3-1Crosscutting Conceptscause And Effectdisciplinary Core Ideashs Ls1.A: Structure And Functionscience And Engineering Practicesconstructing Explanations And Designing Solutionslearning Objectivesexplain How One Organism Can Be Genetically Engineered To Produce A Protein From A Different Organism.Draw Connections Between The Techniques Performed In This Laboratory And The Development Of Current Critical Pharmaceuticals.Explain The Use And Importance Of Selective Marker Genes In A Bacterial Transformation.Prerequisite Knowledge And Skillsstudents Should Have A Basic Knowledge Of Cell Structure And Function, Cell Wall And Membrane Structure And Function, And A Working Knowledge Of Protein Synthesis.Care Guide: Bacteriapurification Of Green Flourscent Protein : Sample Teacher'S Manual