$ 734.16
Details
Lambda DNA digested with PvuI, 0.7% agarose, 3 cleavage sitesconventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'..C G A T▵C G...3'3'..G C▵T A G C...5'Conditions for 100% Activity 1X Buffer R:10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA Incubate at 37°CStorage Buffer PvuI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and Recleavage After 50-fold overdigestion with PvuI, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recutMethylation Effects Dam: completely overlaps —no effect Dcm: never overlaps —no effect CpG: completely overlaps —blocked EcoKI: never overlaps —no effect EcoBI: never overlaps —no effectDigestion of Agarose-embedded DNA Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hoursCompatible Ends Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI
