$ 132.24
Details
Lambda DNA digested with PvuII, 0.7% agarose, 15 cleavage sitesconventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.5'...C A G▵C T G...3'3'...G T C▵G A C...5'Conditions for 100% Activity1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSAIncubate at 37°CStorage BufferPvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and RecleavageAfter 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recutMethylation EffectsDam: never overlaps —no effectDcm: never overlaps —no effectCpG: never overlaps —no effectEcoKI: never overlaps —no effectEcoBI: may overlap —effect not determinedDigestion of Agarose-embedded DNAMinimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours Note:Greater than 15-fold overdigestion with PvuII may result in star activity.
